Search for: All records

Despite the great promise of genetic code expansion technology to modulate structures and functions of proteins, external addition of ncAAs is required in most cases and it often limits the utility of genetic code expansion technology, especially to noncanonical amino acids (ncAAs) with poor membrane internalization. Here, we report the creation of autonomous cells, both prokaryotic and eukaryotic, with the ability to biosynthesize and genetically encode sulfotyrosine (sTyr), an important protein post-translational modification with low membrane permeability. These engineered cells can produce site-specifically sulfated proteins at a higher yield than cells fed exogenously with the highest level of sTyr reported in the literature. We use these autonomous cells to prepare highly potent thrombin inhibitors with site-specific sulfation. By enhancing ncAA incorporation efficiency, this added ability of cells to biosynthesize ncAAs and genetically incorporate them into proteins greatly extends the utility of genetic code expansion methods.

 

Human mobility studies have become increasingly important and diverse in the past decade with the support of social media big data that enables human mobility to be measured in a harmonized and rapid manner. However, what is less explored in the current scholarship is episodic mobility as a special type of human mobility defined as the abnormal mobility triggered by episodic events excess to the normal range of mobility at large. Drawing on a large-scale systematic collection of 1.9 billion geotagged Twitter data from 2017 to 2020, this study contributes to the first empirical study of episodic mobility by producing a daily Twitter census of visitors at the U.S. county level and proposing multiple statistical approaches to identify and quantify episodic mobility. It is followed by four case studies of episodic mobility in U.S. national wide to showcase the great potential of Twitter data and our proposed method to detect episodic mobility subject to episodic events that occur both regularly and sporadically. This study provides new insights on episodic mobility in terms of its conceptual and methodological framework and empirical knowledge, which enriches the current mobility research paradigm. 

Introduction Widespread problems of psychological distress have been observed in many countries following the outbreak of COVID-19, including Australia. What is lacking from current scholarship is a national-scale assessment that tracks the shifts in mental health during the pandemic timeline and across geographic contexts. Methods Drawing on 244 406 geotagged tweets in Australia from 1 January 2020 to 31 May 2021, we employed machine learning and spatial mapping techniques to classify, measure and map changes in the Australian public’s mental health signals, and track their change across the different phases of the pandemic in eight Australian capital cities. Results Australians’ mental health signals, quantified by sentiment scores, have a shift from pessimistic (early pandemic) to optimistic (middle pandemic), reflected by a 174.1% (95% CI 154.8 to 194.5) increase in sentiment scores. However, the signals progressively recessed towards a more pessimistic outlook (later pandemic) with a decrease in sentiment scores by 48.8% (95% CI 34.7 to 64.9). Such changes in mental health signals vary across capital cities. Conclusion We set out a novel empirical framework using social media to systematically classify, measure, map and track the mental health of a nation. Our approach is designed in a manner that can readily be augmented into an ongoing monitoring capacity and extended to other nations. Tracking locales where people are displaying elevated levels of pessimistic mental health signals provide important information for the smart deployment of finite mental health services. This is especially critical in a time of crisis during which resources are stretched beyond normal bounds. 

Studies on human mobility have a long history with increasingly strong interdisciplinary connections across social science, environmental science, information and technology, computer science, engineering, and health science. However, what is lacking in the current research is a synthesis of the studies to identify the evolutional pathways and future research directions. To address this gap, we conduct a systematic review of human mobility-related studies published from 1990 to 2020. Drawing on the selected publications retrieved from the Web of Science, we provide a bibliometric analysis and network visualisation using CiteSpace and VOSviewer on the number of publications and year published, authors and their countries and afflictions, citations, topics, abstracts, keywords, and journals. Our findings show that human mobility-related studies have become increasingly interdisciplinary and multi-dimensional, which have been strengthened by the use of the so-called ‘big data’ from multiple sources, the development of computer technologies, the innovation of modelling approaches, and the novel applications in various areas. Based on our synthesis of the work by top cited authors we identify four directions for future research relating to data sources, modelling methods, applications, and technologies. We advocate for more in-depth research on human mobility using multi-source big data, improving modelling methods and integrating advanced technologies including artificial intelligence, and machine and deep learning to address real-world problems and contribute to social good.